Tim Clausen

Rational correction of pathogenic conformational defects in HTRA1.

Convergence of orphan quality control pathways at a ubiquitin chain-elongating ligase

Homo-BacPROTAC-induced degradation of ClpC1 as a strategy against drug-resistant mycobacteria.

Multiple ubiquitin ligases protect human genome integrity by targeting cancer-associated APOBEC3 deaminases for degradation

TRIM52 is a primate-specific player in the DNA repair process under tight proteolytic control by a triad of giant E3 ligases

UNC-45 assisted myosin folding depends on a conserved FX3HY motif implicated in Freeman Sheldon Syndrome.

Structural basis for regulation of apoptosis and autophagy by the BIRC6/SMAC complex.

Clp-targeting BacPROTACs impair mycobacterial proteostasis and survival.

Shuffled ATG8 interacting motifs form an ancestral bridge between UFMylation and autophagy.

HUWE1 controls tristetraprolin proteasomal degradation by regulating its phosphorylation.

Targeted protein degradation: from small molecules to complex organelles-a Keystone Symposia report.

Potent Bactericidal Antimycobacterials Targeting the Chaperone ClpC1 Based on the Depsipeptide Natural Products Ecumicin and Ohmyungsamycin A.

Moyamoya disease: diagnosis and interventions.

BacPROTACs mediate targeted protein degradation in bacteria.

An allosteric HTRA1-calpain 2 complex with restricted activation profile.

E3 ubiquitin ligase RNF213 employs a non-canonical zinc finger active site and is allosterically regulated by ATP

The oxidoreductase PYROXD1 uses NAD(P)+ as an antioxidant to sustain tRNA ligase activity in pre-tRNA splicing and unfolded protein response.

HUWE1 employs a giant substrate-binding ring to feed and regulate its HECT E3 domain.

McsB forms a gated kinase chamber to mark aberrant bacterial proteins for degradation.

The linear ubiquitin chain assembly complex (LUBAC) generates heterotypic ubiquitin chains.

Multiplexed detection of SARS-CoV-2 and other respiratory infections in high throughput by SARSeq.

Moyamoya disease factor RNF213 is a giant E3 ligase with a dynein-like core and a distinct ubiquitin-transfer mechanism.

Native Mass Spectrometry Can Effectively Predict PROTAC Efficacy.

Activation by substoichiometric inhibition.

A cross-kingdom conserved ER-phagy receptor maintains endoplasmic reticulum homeostasis during stress.

Molecular features of the UNC-45 chaperone critical for binding and folding muscle myosin.

Shaping Striated Muscles with Ubiquitin Proteasome System in Health and Disease.

Structure of McsB, a protein kinase for regulated arginine phosphorylation.

UFD-2 is an adaptor-assisted E3 ligase targeting unfolded proteins.

The crystal structure of Deg9 reveals a novel octameric-type HtrA protease.

Structural mechanism for the recognition and ubiquitination of a single nucleosome residue by Rad6-Bre1.

Structural basis for the disaggregase activity and regulation of Hsp104.

Chemical Biology Interrogates Protein Arginine Phosphorylation.

Arginine phosphorylation marks proteins for degradation by a Clp protease.

Determinants of amyloid fibril degradation by the PDZ protease HTRA1.

The Nuclear Pore-Associated TREX-2 Complex Employs Mediator to Regulate Gene Expression.

RNA specificity and regulation of catalysis in the eukaryotic polynucleotide kinase Clp1.

Myosin chaperones.

Human CLP1 mutations alter tRNA biogenesis, affecting both peripheral and central nervous system function.

Versatile in vitro system to study translocation and functional integration of bacterial outer membrane proteins.

Quantitative phosphoproteomics reveals the role of protein arginine phosphorylation in the bacterial stress response.

Chasing Phosphoarginine Proteins: Development of a Selective Enrichment Method Using a Phosphatase Trap.

Structural basis for recognizing phosphoarginine and evolving residue-specific protein phosphatases in gram-positive bacteria.

The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans.

A structural basis for kinetochore recruitment of the Ndc80 complex via two distinct centromere receptors.

CtpB assembles a gated protease tunnel regulating cell-cell signaling during spore formation in Bacillus subtilis.

Mutations in the β-tubulin gene TUBB5 cause microcephaly with structural brain abnormalities.

Stress-induced GSK3 regulates the redox stress response by phosphorylating glucose-6-phosphate dehydrogenase in Arabidopsis.

Newly folded substrates inside the molecular cage of the HtrA chaperone DegQ.

Human high temperature requirement serine protease A1 (HTRA1) degrades tau protein aggregates.

HTRA proteases: regulated proteolysis in protein quality control.

Augmenting β-augmentation: structural basis of how BamB binds BamA and may support folding of outer membrane proteins.

Structural adaptation of the plant protease Deg1 to repair photosystem II during light exposure.

Protein quality control in the bacterial periplasm.

Molecular adaptation of the DegQ protease to exert protein quality control in the bacterial cell envelope.

Substrate-induced remodeling of the active site regulates human HTRA1 activity.

Conversion of a regulatory into a degradative protease.

HtrA proteases have a conserved activation mechanism that can be triggered by distinct molecular cues.

Molecular transformers in the cell: lessons learned from the DegP protease-chaperone.

Structural basis of substrate specificity of plant 12-oxophytodienoate reductases.

McsB is a protein arginine kinase that phosphorylates and inhibits the heat-shock regulator CtsR.

Peptidic small molecule activators of the stress sensor DegS.

Selectivity profiling of DegP substrates and inhibitors.

Stoichiometry determination of the MP1-p14 complex using a novel and cost-efficient method to produce an equimolar mixture of standard peptides.

Structure, function and regulation of the conserved serine proteases DegP and DegS of Escherichia coli.

Interplay of PDZ and protease domain of DegP ensures efficient elimination of misfolded proteins.

Structural basis for the regulated protease and chaperone function of DegP.

Allosteric activation of HtrA protease DegP by stress signals during bacterial protein quality control.

Regulation of the sigmaE stress response by DegS: how the PDZ domain keeps the protease inactive in the resting state and allows integration of different OMP-derived stress signals upon folding stress.

Crystal structure of 12-oxophytodienoate reductase 3 from tomato: self-inhibition by dimerization.

The role of human HtrA1 in arthritic disease.

Implications of the serine protease HtrA1 in amyloid precursor protein processing.

Molecular machines for protein degradation.

The 1.3 A crystal structure of the flavoprotein YqjM reveals a novel class of Old Yellow Enzymes.

TIS7 regulation of the beta-catenin/Tcf-4 target gene osteopontin (OPN) is histone deacetylase-dependent.

Proteolysis as a regulatory mechanism.

Structural and functional impairment of an Old Yellow Enzyme homologue upon affinity tag incorporation.

Crystal structure of the p14/MP1 scaffolding complex: how a twin couple attaches mitogen-activated protein kinase signaling to late endosomes.

Muscle regeneration and myogenic differentiation defects in mice lacking TIS7.

Crystal structure of the DegS stress sensor: How a PDZ domain recognizes misfolded protein and activates a protease.

Molecular shredders: how proteasomes fulfill their role.

Snapshots of the cystine lyase C-DES during catalysis. Studies in solution and in the crystalline state.

Spectroscopic and kinetic analyses reveal the pyridoxal 5'-phosphate binding mode and the catalytic features of Treponema denticola cystalysin.

The HtrA family of proteases: implications for protein composition and cell fate.

Structure and function of threonine synthase from yeast.

Enzyme-ligand complexes of pyridoxine 5'-phosphate synthase: implications for substrate binding and catalysis.

Crystal structure of DegP (HtrA) reveals a new protease-chaperone machine.

X-ray structure of 12-oxophytodienoate reductase 1 provides structural insight into substrate binding and specificity within the family of OYE.

The three-dimensional structure of cystathionine beta-lyase from Arabidopsis and its substrate specificity.

Structural basis for the function of pyridoxine 5'-phosphate synthase.

Crystal structure of transcription factor MalT domain III: a novel helix repeat fold implicated in regulated oligomerization.

Crystal structures of cystathionine gamma-synthase inhibitor complexes rationalize the increased affinity of a novel inhibitor.

Crystal structure of the cystine C-S lyase from Synechocystis: stabilization of cysteine persulfide for FeS cluster biosynthesis.

X-ray structure of MalY from Escherichia coli: a pyridoxal 5'-phosphate-dependent enzyme acting as a modulator in mal gene expression.

Crystallization and preliminary X-ray crystallographic analysis of PdxJ, the pyridoxine 5'-phosphate synthesizing enzyme.

Crystal structure of a NifS-like protein from Thermotoga maritima: implications for iron sulphur cluster assembly.

Crystal structure of cystalysin from Treponema denticola: a pyridoxal 5'-phosphate-dependent protein acting as a haemolytic enzyme.

Specific inhibition of transsulfuration enzymes.

A new mechanism for the control of a prokaryotic transcriptional regulator: antagonistic binding of positive and negative effectors.

Cloning, purification and characterisation of cystathionine gamma-synthase from Nicotiana tabacum.

Characterization of recombinant Arabidopsis thaliana threonine synthase.

Kinetics and inhibition of recombinant human cystathionine gamma-lyase. Toward the rational control of transsulfuration.

The crystal structure of cystathionine gamma-synthase from Nicotiana tabacum reveals its substrate and reaction specificity.

Crystal structure of Escherichia coli cystathionine gamma-synthase at 1.5 A resolution.

Slow-binding inhibition of Escherichia coli cystathionine beta-lyase by L-aminoethoxyvinylglycine: a kinetic and X-ray study.

Mode of action of cystathionine beta-lyase.

Glutaconate CoA-transferase from Acidaminococcus fermentans: the crystal structure reveals homology with other CoA-transferases.

Cloning, purification, crystallization, and preliminary X-ray diffraction analysis of cystathionine gamma-synthase from E. coli.

Crystal structure of the pyridoxal-5'-phosphate dependent cystathionine beta-lyase from Escherichia coli at 1.83 A.

Cloning, purification, and crystallization of Escherichia coli cystathionine beta-lyase.

Involvement of Tyr24 and Trp108 in substrate binding and substrate specificity of glycolate oxidase.